Catalog Number - 70000-25P

The 250 mm column length provides a substantial increase in theoretical plates and peak capacity compared to shorter columns, enabling:

• Maximum resolution of closely related or co‑eluting polar compounds

• Improved separation confidence in complex sample matrices

• Enhanced method robustness for regulated or validated workflows

The 4.6 mm internal diameter maintains traditional analytical‑scale operation, offering:

• Higher sample loading capacity

• Compatibility with standard injection volumes and UV detectors

• Straightforward integration with existing HPLC infrastructure

This configuration is ideal when separation quality is the top priority and run time is a secondary consideration.

Diamond Hydride™ Stationary Phase Advantages

Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase delivers distinct advantages over conventional bonded‑silica HILIC phases:

• Faster equilibration, even at high organic content

• Stable and reproducible retention mechanisms, reducing method variability

• Long column lifetime, supporting routine and long‑term analytical programs

The silica hydride surface supports mixed‑mode interactions that enhance selectivity for difficult analytes.

This column is routinely selected for:

• Small, highly polar molecules

• Amino acids and organic acids

• Polar and non‑polar peptides

• Metabolites and other challenging biomolecules

Enhanced Separation for Demanding Methods

The extended 250 mm bed length is especially beneficial when:

• Shorter columns do not provide sufficient resolution

• Additional retention time is required for peak discrimination

• Methods demand maximum confidence in peak purity and identity

This makes the 70000‑25P a strong choice for research‑grade HILIC methods, regulated assays, and advanced method development.

Detection Flexibility

While commonly paired with UV and conventional detectors, the 70000‑25P is also compatible with LC‑MS workflows where standard analytical flow rates are acceptable or where post‑column splitting is employed.

System Requirements

• Typical operating flow rates: ~0.8–1.5 mL/min, depending on mobile‑phase viscosity and method conditions

• Compatible with all conventional analytical HPLC systems

• No special low‑flow or UHPLC‑specific pump capability required

Flow rates may be optimized further based on desired resolution, pressure limits, and detector configuration.